The Gut-Brain Axis and Your Microbiome

The microbiota-gut-brain axis links gut microbes with mood and stress. Learn how a comprehensive stool test profiles the microbiome behind it.

Educational overview · Medically reviewed by Madison Ordway, FDN-P

Key facts

  • What it is: two-way communication between gut microbes and the brain.
  • Channels: the vagus nerve, microbial metabolites, immune and hormonal signalling.
  • GI-MAP's role: profiles the gut microbiome relevant to the axis; it does not diagnose mood or neurological conditions.
  • Status: an active, emerging area of science.

The gut-brain axis is the two-way communication network between the gut, its microbes, and the brain. Research increasingly describes a distinct microbiota-gut-brain axis, in which the trillions of microbes in the gut influence — and are influenced by — the nervous system. A comprehensive stool test like the GI-MAP (Gastrointestinal Microbial Assay Plus) profiles the microbiome that sits at the centre of this axis.

What the science says

A comprehensive review of the microbiota-gut-brain axis describes how gut microbes communicate with the brain through multiple routes — the vagus nerve, microbial metabolites, immune signalling and gut hormones — and how this axis is being studied in relation to stress, mood and behaviour (PMID: 31460832).

This is a fast-moving research field. The associations are real and important, but they do not mean a stool test can diagnose or treat a mood or neurological condition.

How testing fits in

Because the axis begins with the microbiome, some people and practitioners use a comprehensive stool profile to understand the beneficial flora, dysbiosis and gut-function markers that research links to gut-brain communication. Any findings are educational inputs, interpreted with a qualified practitioner, and are not a substitute for mental-health care.

The GI-MAP™ reports this and 85+ other markers from one at-home sample.

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References

  1. Cryan JF, O’Riordan KJ, Cowan CSM, et al. The microbiota-gut-brain axis. Physiol Rev. 2019;99(4):1877–2013. PMID: 31460832.

Medically reviewed by

Madison Ordway, FDN-P

Functional Diagnostic Nutrition Practitioner specializing in gut health, hormone balance and mineral optimization. Madison uses GI-MAP testing in her work with clients and has been featured in US Insider, Women’s Journal and The Science Times. See press features →

Content reviewed against Diagnostic Solutions Laboratory documentation and peer-reviewed literature. Last reviewed 6 July 2026.

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